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EpiQuik组蛋白甲基转移酶活性/抑制检测试剂盒(H3K27)

生物耗材网:bioconsumable.com | 更新时间:2016-5-31

产品名称:EpiQuik组蛋白甲基转移酶活性/抑制检测分析试剂盒(H3K27),EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27)

产品货号:P-3005

规格:48次、96次分析

产品简介:

EpiQuik组蛋白甲基转移酶活性/抑制检测试剂盒(H3K27)是方便实验人员专门检测组蛋白H3第27位赖氨酸(H3-K27)的组蛋白甲基转移酶活性/抑制分析的一整套工具。该试剂盒即时使用,提供全部必需的试剂用于成功进行组蛋白甲基转移酶活性/抑制性试验,不接触放射性物质或任何特殊设备。

试剂盒具有如下优点:

l  快速操作,3小时内可完成。

l  安全独创的比色测定,不接触放射性物质,无需提取和层析分析。

l  专一性检测H3-K27组蛋白甲基转移酶活性/抑制。

l  可剥离的微孔板让实验人员灵活选择手工或高通量分析。

l  极其简便,结果可靠,一致的分析条件。

背景信息:

EpiQuik组蛋白甲基转移酶活性/抑制检测试剂盒(H3K27)是为了专门检测组蛋白H3第27位赖氨酸处组蛋白甲基转移酶活性而设计的。在该试剂盒在实际检测中,组蛋白底物紧密结合在微孔表面,组蛋白甲基化转移酶把腺苷基蛋氨酸(Adomet)的一个甲基基团转移到组蛋白H3基质的第27个赖氨酸上将其甲基化。甲基化的H3-K27能被一种高亲和性抗体识别,其数量与组蛋白甲基转移酶活性成线性关系,并且可通过HRP偶联的二抗显色系统进行定量。因此,组蛋白甲基转移酶活性可以依据其所转换的甲基化H3-K27的数量进行计算。

剂盒组分:

HM1 (10X 冲洗缓冲液)

HM2 (组蛋白检测缓冲液)

HM3 (腺苷基蛋氨酸)*

HM4 (生物素标记的底物, 25 µg/ml)*

HM5 (组蛋白甲基转移酶标准品, 10 µg/ml)*

HM6 (结合抗体, 100 µg/ml)*

HM7 (检测抗体, 200 µg/ml)*

HM8 (显色溶液)

HM9 (反应终止溶液)

对照酶样品 (100 µg/ml)*

8孔检测板 (带96孔框架)

产品应用案例:

Veazey KJ et. al. (September 2015). Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects. Epigenetics Chromatin. 8:39.

Zhang J et. al. (September 2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nat Med.

Wan J et. al. (April 2015). PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic Acids Res. 43(7):3591-604.

De la Cruz-Hernandez E et. al. (February 2015). Ribavirin as a tri-targeted antitumor repositioned drug. Oncol Rep.

Liu J et. al. (January 2015). Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 35(1):352-65.

Morishita M et. al. (December 2014). In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L. BMC Struct Biol. 14(1):25.

Malmgren S et. al. (April 2013). Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity. J Biol Chem. 288(17):11973-87.

Latrasse D et. al. (March 2013). Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator. Nucleic Acids Res. 41(5):2907-17.

Li Y et. al. (January 2013). Epigenetic regulation of multiple tumor-related genes leads to suppression of breast tumorigenesis by dietary genistein. PLoS One. 8(1):e54369.

Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.

Rugg-Gunn PJ et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci U S A. 107(24):10783-90.

Nakade K et. al. (April 2009). JDP2 (Jun Dimerization Protein 2)-deficient mouse embryonic fibroblasts are resistant to replicative senescence. J Biol Chem. 284(16):10808-17.

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