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CRISPR/Cas9小鼠单克隆抗体--Epigentek新品

生物耗材网:bioconsumable.com | 更新时间:2015-3-19
CRISPR/Cas9小鼠单克隆抗体(货号:A-9000) ,Epigentek公司最新产品CRISPR/Cas9小鼠单克隆抗体,北京启维益成公司代理CRISPR/Cas9小鼠单克隆抗体,欢迎来电咨询订购 订购电话400 810 0506!
 
背景知识
CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats)是最新出现的一种由RNA指导的Cas9核酸酶对靶向基因进行编辑的技术。CRISPR/Cas9是细菌和古细菌为应对病毒和质粒不断攻击而演化来的获得性免疫防御机制。在这一系统中,crRNA(CRISPR-derived RNA)通过碱基配对与tracrRNA(trans-activating RNA)结合形成双链RNA,此tracrRNA/crRNA二元复合体指导Cas9蛋白在crRNA引导序列靶定位点切断双链DNA。在基因组编辑过程中,tracrRNA和crRNA可以融合成为1条RNA(sgRNA)表达同样可以起到靶向剪切的作用。CRISPR/Cas9的优点是操作简单,对基因组的效率高。需要对某一个靶位点编辑的时候,只需要表达相应的sgRNA即可,不需要对Cas9核酸酶进行改造。它可对任何物种的基因组进行高效率的定向编辑。
浓度:1mg/ml
描述:小鼠单克隆抗体用于检测CRISPR/Cas9,克隆号:7A9,抗原来自于链球菌(S. pyogenes)CRISPR-相关的核酸内切酶Cas9/Csn1相应序列的合成肽,此抗体可通过WB,IF,IP或ELISA方法检测CRISPR/Cas9在靶细胞中的表达以确认和验证gRNA和Cas9载体否成功转染。
特异性:能同时识别Cas9和dCas9(nuclease deficient Cas9)
反应性:Species Independent
同型对照:IgG/Kappa
成份:PBS, 30% 甘油
储存:4℃可短期保存1-2周,若长期储存,可分成等份保存于-20℃,避免反复冻融。
纯化:蛋白G纯化
操作建议:为最大量地回收产品,打开之前先离心一下产品瓶。
别名:Anti-Cas9,Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody,Cas9 7A9
应用
Western Blot: 1:1000
Immunofluorescence: 1:500
IP: 2ug/106 cells
ELISA
ChIP应用,推荐用Cas9多克隆抗体(货号:A-1111)
 
Fig. 1. Western Blot: Shown are the results of WB on protein extracts from untransfected (A) and transfected (B) HEK293 cells using the Anti-CRISPR-Cas9 mAb.
Fig. 2. Immunofluorescence: Hela cells were transiently transfected with an N-terminally Flag-tagged S. pyogenes Cas9 expression vector. The cells were stained with the Anti-Cas9 monoclonal antibody followed by anti mouse-AF488 coupled secondary antibody. Nuclei were counter-stained with Hoechst 33342.
Fig. 3. Immunoprecipitation: HEK293T expressing N-terminally Flag-tagged S.pyogenes Cas9 were lysed 72 hours post transfection. Proteins were immunoprecipitated from 100 μg of whole cell lysate for 1H at 4°C with Cas9 Sup followed by incubation for 1h at 4°C with a 1:1 mixture of protein A/G sepharose beads, or for 2H at 4°C with the Cas9 mAb X-linked to a 1:1 mixture of protein A/G sepharose beads. Beads were washed and eluted by boiling in Laemmli, separated by SDS-PAGE, and transferred to nitrocellulose. Membrane was blocked, incubated with the Cas9 monoclonal ab, then incubated with HRP anti-mouse antibody.
 
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