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重组小鼠白血病抑制因子 (mLIF)产品促销

生物耗材网:bioconsumable.com | 更新时间:2015-6-18

惊爆价促销 重组小鼠白血病抑制因子 (mLIF)

促销日期截止:20159月底

 

LIF, mouse, recombinant
重组小鼠LIF
货号:01-1140
LOT:01-1-114-9111
规格:10ug
产品描述
LIF(白血病抑制因子,骨髓性白血病抑制因子)是一种多效性淋巴因子,它最初被描述为髓系白血病细胞增殖的抑制因子和髓系白血病细胞分化为巨噬细胞的诱导因子。LIF的其他活性包括抑制脂肪形成和胆碱能神经元的分化和骨代谢。人和小鼠的LIF在氨基酸水平上有78%的同源性,且LIF的活性是非物种特异性。人的LIF在小鼠的细胞是有活性的,但鼠源动物的LIF作用于人的细胞是没有活性的。
来源
ISOkine™重组小鼠LIF产生于大麦籽粒的胚乳组织中。
组分
PBS,pH7.2,已滤灭
纯度
通过SDS-PAGE凝胶分析纯度大于95%
溶解
注意:打开之前务必先离心,建议在灭菌水中溶解冻干粉蛋白以浓度不低于100ug/ml。若长期储存建议添加载体蛋白(0.1%HAS或BSA)。
稳定性
冻干粉蛋白在室温下能稳定保存数周,但最好保存于-20℃。溶解的蛋白最好现配现用或工作液分成等份儿保存于-20℃。避免反复冻融。
生物活性
每一批的ISOkine™生长因子都被测试和证实和市售来源产品有相似的活性。ISOkine™重组鼠源LIF的生物活性通过其诱导鼠源M1骨髓系白血病细胞的能力来决定,50U的定义是诱导50%的M1细胞的分化所需求的mLIF的量。10ug的ISOkine™ mLIF蛋白大约等同于10e6活性单位。为得到小鼠基因敲除,ISOkine™ mLIF已在标准的以mESC为基础的基因打靶技术制备基因敲除小鼠模型实验中验证过。具体应用的最佳浓度,我们建议通过初始剂量反应分析来决定。
内毒素水平
每ug ISOkine™产品的内毒素水平低于0.005ng(< 0.05EU/µg)。
MAT分析
纯化的ISOkine™系列产品不携带有致热的或促炎污染物,因为用人10-plex细胞因子结合单核细胞活化测试分析检测IL-6, TNF-alpha 和IL-1beta的诱导. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland。
分子量
重组小鼠LIF包含了188个氨基酸和一个长度为10个氨基酸组氨酸标签,总氨基酸数为198,预测分子量为21.2kDa。由于糖基化,重组蛋白在SDS-PAGE电泳下迁移显示分子量大小为25-30 kDa。
 
2011-2015年使用mLIF所发表的文献:
Toni Pfaffeneder, Fabio Spada, Mirko Wagner, Caterina Brandmayr, Silvia Laube, David Eisen,Matthias Truss, Jessica Steinbacher, Benjamin Hackner, et al. Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA.
Nature Chemical Biology. 2014. Vol 10, Issue 3, p. 574-581
 
Vinod Verma, Ben Huang, Prasanna Kumar Kallingappa, Björn Obac. Dual Kinase
Inhibition Promotes Pluripotency in Finite Bovine Embryonic Cell Lines.
Stem Cells and Development. 2013. Vol 22, Issue 11, p. 1728-1742

David J. Pearton, Craig S. Smith, Emma Redgate, Jessica van Leeuwen, Martyn Donnison, Peter L. Pfeffer. Elf5 counteracts precocious trophoblast differentiation by maintaining Sox2 and 3 and inhibiting Hand1 expression.
Developmental Biology. 2014. Vol 392, p. 344-357

Rie Horiuchi, Takayuki Akimoto, Zhang Hong, Takashi Ushida. Cyclic mechanical strain maintains Nanog expression through PI3K/Akt signaling in mouse embryonic stem cells.
Experimental Cell Research. 2012. Vol 318, p. 1726–1732

Bruno Di Stefano, Thomas Graf. Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse Pre-B Cells.
Methods in Molecular Biology. 2014. ePublication ahead of print

Priscila Ramos-Ibeas, Eva Pericuesta, Raul Fernandez-Gonzalez, Alfonso Gutierrez-Adan, Miguel Angel Ramirez. Germ-Cell Culture Conditions Facilitate the Reprogramming to Produce Mouse Embryonic Stem Cells.
Molecular Reproduction and Development. 2014 Vol 81, Issue 9, p. 794-804
Nina Schönhuber, Barbara Seidler, Kathleen Schuck, Christian Veltkamp, Christina Schachtler, Magdalena Zukowska, Stefan Eser, Thorsten B Feyerabend, Mariel C Paul, Philipp Eser, Sabine Klein, Andrew M Lowy, Ruby Banerjee, Fangtang Yang, Chang-Lung Lee, Everett J Moding, David G Kirsch, Angelika Scheideler, Dario R Alessi, Ignacio Varela, Allan Bradley, Alexander Kind, Angelika E Schnieke, Hans-Reimer Rodewald, Roland Rad, Roland M Schmid, Günter Schneider, Dieter Saur. A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.
Nature Medicine. 2014. Vol 20, Issue 11, p. 1340-1347.
 
Klein S, Seidler B, Kettenberger A, Sibaev A, Rohn M, Feil R, Allescher HD, Vanderwinden JM, Hofmann F, Schemann M, Rad R, Storr MA, Schmid RM, Schneider G, and Saur D. Interstitial cells of Cajal integrate excitatory and inhibitory neurotransmission with intestinal slow-wave activity.
Nature Communications. 27 March 2013. Article number: 1630

Pawel M. Switonski, Wojciech J. Szlachcic, Wlodzimierz J. Krzyzosiak, Maciej Figiel. A new
humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of
neurons and glia and late disease onset of SCA3/MJD.
Neurobiology of Disease. 2015. Vol 73, p. 174-188.

Deisl C, Simonin A, Anderegg M, Albano G, Kovacs G, Ackermann D, Moch H, Dolci W, Thorens B, Hediger MA, Fuster DG. Sodium/hydrogen exchanger NHA2 is critical for insulin secretion in ß-cells.
Proceedings of the National Academy of Sciences U S A. 2013. Vol 110, Issue 24, p.10004-10009.

Manoj B. Menon, Akihiro Sawada, Anuhar Chaturvedi, Pooja Mishra, Karin Schuster-Gossler,
Melanie Galla, Axel Schambach, Achim Gossler, Reinhold Förster, Michael Heuser, Alexey
Kotlyarov, Makoto Kinoshita, Matthias Gaestel. Genetic Deletion of SEPT7 Reveals a Cell TypeSpecific Role of Septins in Microtubule Destabilization for the Completion of Cytokinesis.
PLOS Genetics. 2014. August.

Sluka SHM, Akhmedov A, Vogel J, Unruh D, Bogdanov VY, Camici GG, Lüscher TF, Ruf W, and Tanner FC. Alternatively Spliced Tissue Factor Is Not Sufficient for Embryonic Development.
PLoS ONE. 2014. May.


Gillet L, Shy D, Sonntag S, Rougier J-S, Verkerk A, Essers M, Marsman R, Shmerling D, Bezzina C,Remme CA, and Abriel H. 0150: PDZ domain proteins interacting with Nav1.5 differentially regulate Nav1.5 channel pools in mouse cardiomyocytes.
Archives of Cardiovascular Diseases. 2014. Vol 6, Supplement 1, p. 40-41.

 

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