ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.
This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.
This dye can replace Alexa Fluor 568.
| Appearance: | dark crimson powder |
| Mass spec M+ increment: | 516.2 |
| Molecular weight: | 631.67 |
| Molecular formula: | C37H33N3O7 |
| CAS number: | 117491-83-5 |
| Solubility: | good in polar organic solvents (DMF, DMSO), low in water |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| Excitation maximum, nm: | 570 |
| Extinction coefficient at excitation maximum, Lmol-1cm-1: | 82000 |
| Emission maximum, nm: | 591 |
| Fluorescence quantum yield: | 1.0 |
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