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【产品名称】
 Human Blood Cell (hBLD) Reprogramming Kit
【订购货号】
 S378440
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2016-10-27  

Description
StemmeraTM Human Blood Cell (hBLD) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for culturing human PBMCs and induction of pluripotent stem cells (hiPSCs) from PBMCs, as well as maintenance of iPSCs under completely serum-free and feeder-free conditions.

Features

  • Ready-to-use for blood cell culture, blood cell reprogramming & freezing of iPSCs
  • One-step reprogramming
  • High efficiency reprogramming
  • Serum/feeder-free culture and maintenance
  • Easy protocol

Product Component

Human Blood Cell Reprogramming Kit-I

  • Cat# ST01005-S1 Non-Enzymatic Cell Dissociation Solution (100ml)
  • Cat# ST01005-S2/BM Blood Cell Reprogramming Basal Medium (2x500ml)
  • Cat# ST01005-S3 DMEM/F12 (60ml)

Human Blood Cell Reprogramming Kit-II

  • Cat# ST01005-S4/BM Blood Cell Basal Medium (2x25ml)
  • Cat# ST01005-S4/S Blood Cell Supplement (61μl for 25ml of Basal Medium)
  • Cat# ST01005-S5 Polybreen (10μl)
  • Cat# ST01005-S6 Matrix (5x100μl)
  • Cat# ST01005-S2/S Blood Cell Reprogramming Supplement (2x3.5ml)
  • Cat# ST01005-S7 Rock Inhibitor (1000x) (500μl)
  • Cat# ST01005-S8 Blood Cell CryopreservationSolution (10ml)
  • Cat# ST01005-S9 Serum-Free Cryopreservation Solution for hPSC (20ml)

Human Blood Cell Reprogramming Kit-III

  • Cat# ST01005-S10-24 Cell Culture Plates (1x24 well)
  • Cat# ST01005-S10-12 Cell Culture Plates(1x12 well)
  • Cat# ST01005-S10-6 Cell Culture Plates(4x6 well)
  • Cat# ST01005-S11 Cell Counter Slides (3pcs)
  • Cat# ST01005-S12 Trypan Blue (300μl)
  • Reprogramming Factors (Not supplied)

Note: Sendai virus reprogramming factors from Life Technologies Inc is strongly recommended, Cat # A16517

Storage & Handling
Store all components as directed in manual instruction upon arrival and until the expiration dates stated on the product label.Avoid multiple freeze/thaw cycles. The complete medium should remain stable for 4-6 weeks at 2-8°C. Make aliquot of complete medium for daily use, avoid warm up cycles. Protect from light is preferred.

Product Use
This product is intended for in vitro use and research use only. Not intended for human or animal diagnostic or therapeutic uses.

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