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【产品名称】
 EpiQuik植物染色质免疫沉淀试剂盒
【订购货号】
 E14529
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2015-8-26  

产品名称:植物染色质免疫沉淀试剂盒,EpiQuik Plant ChIP Kit

产品货号:P-2014

产品规格:24 reactions,48 reactions

产品简介

EpiQuik™植物染色质免疫沉淀试剂盒包括全套的试剂,允许试验者有效地在体内研究蛋白-DNA相互关系。整个过程可以在6小时内完成,产品效果远远优于任何竞争对手的试剂盒。EpiQuik™植物染色质免疫沉淀试剂盒适用于将特异性免疫沉淀与定性和定量PCR、ChIP-Seq、ChIP-on-chip结合使用。

这试剂盒具有如下优点:   

快速和简单的程序,实验能在6小时内完成。

微孔板的形式使得分析灵活:手动或高通量。

包括DNA纯化柱:节省时间和减少劳动力。

与所有的DNA扩增方法兼容。

能达到简单、可靠和一致的检测条件。

背景信息:

蛋白质-DNA相互作用在细胞功能,如信号转导,基因转录,染色体分离,基因复制和重组,和表观遗传沉默中发挥了关键作用。在植物中,DNA结合蛋白和同源启动子序列之间的相互作用建立了基因的时间和空间的表达模式,并影响了体内的平衡,发育和适应性。染色质免疫沉淀(ChIP)识别特定的调节蛋白及其靶基因的全基因组关联之间的直接提供了一个有利的工具。不像其他的方法如EMASA,DNA微阵列,报告基因检测,是在体外分析DNA和蛋白质的相互作用,染色质免疫沉淀(ChIP)可以在活细胞中检测特定蛋白结合到一个特定的基因序列。

程序:

该试剂盒包括从植物细胞中成功实施染色质免疫沉淀所需要的所有试剂。特别是,该试剂盒包括一个ChIP级二甲基组蛋白H3-K9抗体和正常小鼠IgG的阴性对照。从细胞染色质提取,剪切,添加到抗体封闭的微孔中。抗体捕获蛋白质-DNA复合物后DNA被释放,反转后通过特异设计的快速离心柱纯化。洗脱下来的DNA可用于各种下游应用。

起始材料:

起始材料可包括各种植物组织(花、叶、幼苗)。在一般情况下,每个反应输入量应该是从20到50毫克的植物组织。

 

 

  • Fig. 1. Schematic procedure of the EpiQuik™ Plant ChIP Kit.

Fig2.DNA was immunoprecipitated from 2-week-old icu2-1/icu2-1 seedlings. PCR was used to amplify the ORNITHINE TRANSCARBAMILASE (OTC) gene and regions of the AGAMOUS gene. 
Product Overview


The EpiQuik™ Plant ChIP Kit is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vivo efficiently. The entire procedure can be completed within 6 hours and produces far superior results than any competitor kits. The EpiQuik™ Plant ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ PLANT ChIP KIT?

  • The fastest procedure available, which can be finished within 6 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.
Product Details


The EpiQuik™ Plant ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly, this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

 

 

 

SCHEMATIC PROCEDURE:



DNA was immunoprecipitated from 2-week-old icu2-1/icu2-1 seedlings. PCR was used to amplify the ORNITHINE TRANSCARBAMILASE (OTC) gene and regions of the AGAMOUS gene.

 

 

Product Components


CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3C (5X Lysis Buffer I)
CP3D (Lysis Buffer II)
CP3E (Lysis Buffer III)
CP3F (Lysis Buffer IV)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Normal Mouse IgG (1 mg/ml)*
Anti-Dimethyl H3-K9 (1 mg/ml)*
Proteinase K (10 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS


 

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

 

Product Citations


Kim, JS. et. al. (December 2011). An ABRE Promoter Sequence is Involved in Osmotic Stress-Responsive Expression of the DREB2A Gene, Which Encodes a Transcription Factor Regulating Drought-Inducible Genes in Arabidopsis. Plant Cell Physiol. 52(12):2136-46.PubMed Abstract

Yamaguchi, T. et. al. (August 2010). Cortisol is involved in temperature-dependent sex determination in the Japanese flounder.Endocrinology. 151(8): 3900-8. PubMed Abstract

Guo, Z. et. al. (April 2010). TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. Plant Cell. 22(4): 1161-73. PubMed Abstract

Hirai, S. et. al. (April 2010). Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco. FEBS J. 277(7): 1695-703. PubMed Abstract

Shibuya, K. et. al. (February 2009). RNA-directed DNA methylation induces transcriptional activation in plants. PNAS. 106(5): 1660-5.PubMed Abstract

Aoyama, T. et. al. (January 2008). Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation. Biochemical and Biophysical Reasearch Communities. 365(1): 124-30. PubMed Abstract

Barrero, J. M. et. al. (September 2007). INCURVATA2 Encodes the Catalytic Subunit of DNA Polymerase {alpha} and Interacts with Genes Involved in Chromatin-Mediated Cellular Memory in Arabidopsis thaliana. Plant Cell. 19(9): 2822-38. PubMed Abstract

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