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【产品名称】
 EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Colorimetric)
【订购货号】
 E15687
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Tri-Methyl Histone H3-K27 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K27 QUANTIFICATION KIT (COLORIMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Specifically captures tri-methylated H3-K27 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
  • The control is conveniently included for the quantification of tri-methylated H3-K27.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details

The EpiQuik™ Global Tri-Methyl Histone H3-K27 Quantification Kit (Colorimetric) is designed for measuring global histone H3-K27 tri-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 27 is captured to the strip wells coated with an anti-trimethyl H3-K27 antibody. The captured tri-methylated histone H3-K27 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of tri-methylated H3-K27 is proportional to the intensity of absorbance. The absolute amount of tri-methylated H3-K27 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:  
 
Product Components

 

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide 

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

 

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Neri, F. et. al. (February 2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51. PubMed Abstract

Deligezer, U. et. al. (October 2010). Post-treatment circulating plasma BMP6 mRNA and H3K27 methylation levels discriminate metastatic prostate cancer from localized disease. Clin Chim Acta.  411(19-20): 1452-6. PubMed Abstract

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