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【产品名称】
 EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Fluorometric)
【订购货号】
 E15671
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K9 QUANTIFICATION KIT (FLUOROMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative fluorometric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Specifically captures tri-methyl H3-K9 with the detection limit as low as 0.4 ng/well and detection range from 5 ng - 2 µg/well of histone extracts.
  • The control is conveniently included for the quantification of the amount of tri-methylated H3-K9.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Global Tri-Methyl Histone H3-K9 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K9 tri-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 9 is captured to the strip wells coated with an anti-tri-methyl H3-K9 antibody. The captured tri-methylated histone H3-K9 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of tri-methylated H3-K9 is proportional to the intensity of fluorescence. The absolute amount of tri-methylated H3-K9 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:  
 

 Histone extracts were prepared from MDA-MB-231 cells using the EpiQuik Total Histone Extraction Kit and the amount of trimethyl H3-K9 was measured using the EpiQuik Tri-Methyl Histone H3-K9 Quantification Kit (Fluorometric).

Product Components

 

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detection Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations


 

Terao, M. et. al. (December 2010). Induction of MIR-21 by retinoic acid in estrogen-receptor-positive breast carcinoma cells: biological correlates and molecular targets. J Biol Chem. Epub ahead of print. Full PDF Article

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