北京启维益成科技有限公司 旗下网站

生物耗材网 请登录 | 免费注册 | 联系我们 
  首 页 >> 生物试剂 >> 试剂盒 >> 表观遗传学 >>
【产品名称】
 EpiQuik Global Mono-Methyl Histone H3K36 Quantification Kit (Fluorometric)
【订购货号】
 E15692
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2015-4-7  

Product Overview

The EpiQuik™ Global Mono-Methyl Histone H3-K36 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global mono-methylation of histone H3-K36 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL MONO-METHYL HISTONE H3-K36 QUANTIFICATION KIT (FLUOROMETRIC)?

Quick and efficient procedure, which can be finished within 2.5 hours. Innovative fluorometric assay without the need for radioactivity, electrophoresis, and chromatography. Specifically captures mono-methylated H3-K36 with the detection limit as low as 0.4 ng/well and detection range from 5 ng-2 µg/well of histone extracts. The control is conveniently included for the quantification of the amount of mono-methylated H3-K36. Strip microplate format makes the assay flexible: manual or high throughput. Simple, reliable, and consistent assay conditions. Product Details

The EpiQuik™ Global Mono-Methyl Histone H3-K36 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K36 mono-methylation. In an assay with this kit, the mono-methylated histone H3 at lysine 36 is captured to the strip wells coated with an anti-monomethyl H3-K36 antibody. The captured mono-methylated histone H3-K36 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of mono-methylated H3-K36 is proportional to the intensity of fluorescence. The absolute amount of mono-methylated H3-K36 can be quantified by comparing to the standard control.

SCHEMATIC PROCEDURE:     Product Components

 

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detection Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

打印此页】 【返回】【顶部】【关闭

【相关产品】