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【产品名称】
 EpiQuik Global Mono-Methyl Histone H3K27 Quantification Kit (Colorimetric)
【订购货号】
 E15674
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Mono-Methyl Histone H3-K27 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global mono-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL MONO-METHYL HISTONE H3-K27 QUANTIFICATION KIT (COLORIMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Specifically captures mono-methylated H3-K27 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
  • The control is conveniently included for the quantification of mono-methylated H3-K27.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Global Mono-Methyl Histone H3-K27 Quantification Kit (Colorimetric) is designed for measuring global histone H3-K27 mono-methylation. In an assay with this kit, the mono-methylated histone H3 at lysine 27 is captured to the strip wells coated with an anti-monomethyl H3-K27 antibody. The captured mono-methylated histone H3-K27 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of mono-methylated H3-K27 is proportional to the intensity of absorbance. The absolute amount of mono-methylated H3-K27 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:  
 

 Histone extracts were prepared from MDA-MB-231 cells using the EpiQuik Total Histone Extraction Kit and the amount of monomethyl H3-K27 was measured using the EpiQuik Mono-Methyl Histone H3-K27 Quantification Kit (Colorimetric).

Product Components

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide 

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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