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【产品名称】
 EpiQuik Global Di-Methyl Histone H4K20 Quantification Kit (Fluorometric)
【订购货号】
 E15712
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Di-Methyl Histone H4-K20 Quantification Kit (Fluorometric) is a complete set of essential components which enables the experimenter to measure global di-methylation of histone H4-K20. Di-methylation of H4-K20 has been described as another repressive chromatin domain and is involved in DNA damage response. The global H4-K20 di-methylation can also be changed by inhibiton or activation of HMTs. Thus, quantitative detection of global di-methyl histone H4-K20 would provide useful information for better understanding epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. 

WHY CHOOSE THE EPIQUIK™ GLOBAL DI-METHYLY HISTONE H4-K20 QUANTIFICATION KIT (FLUOROMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Specifically captures di-methylated H4-K20 with the detection limit as low as 1 ng/well and detection range from 10 ng-2 µg/well of histone extracts.
  • The control is conveniently included for the quantification of di-methylated H4-K20.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Global Di-Methyl Histone H4-K20 Quantification Kit(Fluorometric) is designed for measuring global histone H4-K20 di-methylation. In an assay with this kit, the di-methylated histone H4 at lysine 20 is captured to the strip wells coated with an anti-dimethyl H4-K20 antibody. The captured di-methylated histone H4-K20 can then be detected with a detection antibody, followed by a fluorescent development reagent. The ratio of di-methylated H4-K20 is proportional to the intensity of fluorescence. The absolute amount of di-methylated H4-K20 can be quantitated by comparing to the standard control. 

SCHEMATIC PROCEDURE:  

Product Components

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detecting Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
Signal Report Solution*
Signal Enhancer*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide 

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

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