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【产品名称】
 EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
【订购货号】
 E15707
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric) is a complete set of essential components which enables the experimenter to quantify levels of histone H3 proteins independent of their modified states, and it can also be used for normalizing the modified histone H3 content of samples when run in parallel with Epigentek histone modification quantification kit series. The EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric) is suitable for specifically measuring total histone H3 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells. 

WHY CHOOSE THE EPIQUIK™ TOTAL HISTONE H3 QUANTIFICATION KIT?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without need for radioactivity, electrophoresis, and chromatography.
  • Specifically captures histone H3 with the detection limit as low as 10 ng/well and detection range from 20 ng - 1 µg/well of histone extracts.
  • The unmodified histone H3 control is conveniently included for the quantification of the amount of total histone H3.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Total Histone H3 Quantification Kit (Colorimetric) is designed for quantifying levels of histone h3 proteins independent of their modified states. In an assay with this kit, the histone H3 protein is captured to the strip wells coated with an anti-histone H3 antibody. The captured histone H3 can then be detected with a detection antibody followed by a color development reagent. The ratio of histone H3 is proportional to the intensity of absorbance. The absolute amount histone H3 can be quantified by comparing to the standard control. 

SCHEMATIC PROCEDURE:  

Product Components

 

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide 

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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