北京启维益成科技有限公司 旗下网站

生物耗材网 请登录 | 免费注册 | 联系我们 
  首 页 >> 生物试剂 >> 试剂盒 >> 表观遗传学 >>
【产品名称】
 EpiQuik Global Tri-Methyl Histone H3K4 Quantification Kit (Fluorometric)
【订购货号】
 E15663
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Tri-Methyl Histone H3-K4 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global tri-methylation of histone H3-K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL TRI-METHYL HISTONE H3-K4 QUANTIFICATION KIT (FLUOROMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative fluorometric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Specifically captures tri-methyl H3-K4 with the detection limit as low as 0.4 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
  • The control is conveniently included for the quantification of the amount of tri-methylated H3-K4.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Global Tri-Methyl Histone H3-K4 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K4 tri-methylation. In an assay with this kit, the tri-methylated histone H3 at lysine 4 is captured to the strip wells coated with an anti-trimethyl H3-K4 antibody. The captured tri-methylated histone H3-K4 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of tri-methylated H3-K4 is proportional to the intensity of fluorescence. The absolute amount of tri-methylated H3-K4 can be quantitated by comparing to the standard control. 

SCHEMATIC PROCEDURE:  
 

 Histone extracts were prepared from MDA-231 cells using the EpiQuik Total Histone Extraction Kit and the amount of trimethyl-H3-K4 was measured using the EpiQuik Tri-Methyl Histone H3-K4 Quantification kit (Fluorometric).

Product Components

 

 

F1 (10X Wash Buffer)
F2 (Antibody Buffer)
F3 (Detection Antibody, 1 mg/ml)*
F4 (Fluoro Developer)*
F5 (Fluoro Enhancer)*
F6 (Fluoro Dilution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Neri, F. et. al. (February 2012). Myc Regulates the Transcription of the PRC2 Gene To Control the Expression of Developmental Genes in Embryonic Stem Cells. Mol Cell Biol. 32(4):840-51. PubMed Abstract

打印此页】 【返回】【顶部】【关闭

【相关产品】