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【产品名称】
 EpiQuik Global Pan-Methyl Histone H4K20 Quantification Kit (Colorimetric)
【订购货号】
 E15715
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-4-1  
Product Overview

The EpiQuik™ Global Pan-Methyl Histone H4-K20 Quantification Kit (Colorimetric) is a complete set of essential components, which enables the experimenter to measure global mono-, di-, and tri-methylation of histone H4-K20 on a single 96-well plate. The EpiQuik Global Pan-Methyl Histone H4-K20 Quantification Kit (Colorimetric) is suitable for specifically measuring global histone H4-K20 pan-methylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells. 

WHY CHOOSE THE EPIQUIK™ GLOBAL PAN-METHYL HISTONE H4-K20 QUANTIFICATION KIT (COLORIMETRIC)?

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Simultaneously quantify mono-, di-, and tri-methylated H4-K20 with the detection limit as low as 5 ng/well and a detection range from 20 ng-2 µg/well of histone extracts.
  • The control is conveniently included for the quantification of pan-methylated H4-K20.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details


 

The EpiQuik™ Global Pan-Methyl Histone H4-K20 Quantification Kit (Colorimetric) is designed for simultaneously measuring mono-, di-, and tri-methylation of histone H4-K20. In an assay with this kit, the mono-, di-, and tri-methylated histone H4 at lysine 20 is captured to strip wells coated with an anti-mono-, di-, and trimethyl H4-K20 antibody. The captured methylated histone H4-K20 can then be detected with a detection antibody followed by a color development reagent. The ratio of mono-, di-, and tri-methylated H4-K20 is proportional to the intensity of absorbance. The absolute amount of mono-, di-, and tri-methylated H4-K20 can be quantitated by comparing to the standard control. 

SCHEMATIC PROCEDURE:  

Product Components

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detecting Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
Signal Report Solution*
Signal Enhancer*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

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