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【产品名称】
 Methylamp总DNA甲基化定量试剂盒
【订购货号】
 E112891
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2015-3-24  
Product Overview

The Methylamp™ Global DNA Methylation Quantification Ultra Kit is an improved version of the popular Methylamp™ Global DNA Methylation Quantification Kit. As a complete set of essential components, the kit enables the experimenter to quantify global DNA methylation through an ELISA-like reaction using Epigentek's uniquely simplified and streamlined procedure. The entire procedure can be completed in less than 4 hours in either manual or high throughput analysis. In addition to having a shorter procedure, the improved kit also has higher sensitivty than its predecessor. It is suitable for detecting global DNA methylation status using genomic DNA isolated from variety of cultured cells, fresh and frozen tissue, paraffin-embedded tissue, plasma/serum sample, and body fluid sample, etc. The universal positive control included in the kit allows for quantitation of methylated DNA from any species.

WHY CHOOSE THE METHYLAMP™ GLOBAL DNA METHYLATION QUANTIFICATION KIT?

  • Colorimetric quantification without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.


IMPROVEMENTS OVER ITS PREDECESSOR:

  • Shorter procedure, which can be finished in less than 4 hours.
  • Significantly higher sensitivity -- detection limitation can be as low as 0.2 ng of methylated DNA.
  • Universal positive control suitable for quantifying methylated DNA from any species.
Product Details


The Methylamp™ Global DNA Methylation Quantification Ultra Kit contains all reagents required for quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with DNA affinity substance. The methylated fraction of DNA can be recognized by 5-methylcytosine antibody and quantified through an ELISA-like reaction. The amount of methylated DNA is proportional to the OD intensity.

 

 

SCHEMATIC PROCEDURE:



Quantification of Methylated DNA.

 

Product Components


GU1 (10X Wash Buffer)
GU2 (DNA Binding Solution)
GU3 (Positive Control,100 µg/ml)**
GU4 (Block Solution)
GU5 (Capture Antibody, 1000 µg/ml)*
GU6 (Detecting Antibody, 400 µg/ml)*
GU7 (Enhance Solution)
GU8 (Developing Solution)
GU9 (Stop Solution)
NC (Negative Control)
8-Well Assay Strips (with Frame)
User Guide

* Spin the solution down to the bottom before use.
** This control is synthesized polynucleiotide methylated at every 5-cytosine.

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Product Citations


McGuinness, D. et. al. (January 2012). Socio-economic status is associated with epigenetic differences in the pSoBid cohort. Int J Epidemiol. Epub ahead of print. PubMed Abstract

Shearstone, JR. et. al. (November 2011). Global DNA demethylation during mouse erythropoiesis in vivo. Science. 334(6057):799-802 PubMed Abstract

Birkus, G. et. al. (May 2011). Role of cathepsin A and lysosomes in the intracellular activation of novel antipapillomavirus agent GS-9191. Antimicrob Agents Chemother. 55(5)):2166-73 PubMed Abstract

Shi, Y. Y. et. al. (April 2011). Diet and Cell Size Both Affect Queen-Worker Differentiation through DNA Methylation in Honey Bees (Apis mellifera, Apidae). PLoS One. 6(4): e18808. PubMed Abstract

Desplats, P. et. al. (February 2011). Alpha-synuclein sequesters DNMT1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. Epub ahead of print. PubMed Abstract

Huang, S. K. et. al. (November 2010). Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice. Am J Pathol. 177(5): 2245-55. PubMed Abstract

Liu, C. et. al. (November 2010). Global DNA Methylation, DNMT1, and MBD2 in Patients with Systemic Lupus Erythematosus.Lupus. 20(2): 131-6. PubMed Abstract

Vucetic, Z. et. al. (October 2010). Maternal high-fat diet alters methylation and gene expression of dopamine and opioid-related genes. Endocrinology. 151(10): 4756-64. PubMed Abstract

LaPlant, Q. et. al. (September 2010). Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nat Neurosci. 13(9): 1137-43. PubMed Abstract

Sanders, Y. Y. et. al. (August 2010). Epigenetic Regulation of Thy-1 by Histone Deacetylase Inhibitor in Rat Lung Fibroblasts. Am J Respir Cell Mol Biol. Epub ahead of print. PubMed Abstract

Li, Y. et. al. (May 2010). Overexpression of the growth arrest and DNA damage-induced 45 gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells. Arthritis & Rheumatism. 62(5): 1438-1447. PubMed Abstract

Wang, L. et. al. (February 2010). Relation between hypomethylation of long interspersed nucleotide elements and risk of neural tube defects. Am. J. Clin. Nutr. 91(5): 1359-67. Full PDF Article

Yamanaka, K. l. et. al. (February 2010). Gene Silencing of DNA Methyltransferases by RNA Interference in Bovine Fibroblast Cells. J Reprod Dev. 56(1): 60-7. Full PDF Article 

Shutoh, Y. et. al. (October 2009). Low dose effects of dichlorodiphenyltrichloroethane (DDT) on gene transcription and DNA methylation in the hypothalamus of young male rats: implication of hormesis-like effects. Journal of Toxicological Sciences. 34(5): 469-482. PubMed Abstract 


Blazkova, J. et. al. (August 2009). CpG methylation controls reactivation of HIV from latency. PLoS Pathogens. 5(8): e1000554. Full PDF Article 

Chen, C. et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Investigation. 27(5): 582-91. PubMed Abstract

Mahmoud, W. F. et. al. (March 2009). AtMBD9 modulates Arabidopsis development through the
dual epigenetic pathways of DNA methylation and histone acetylation. The Plant Journal. 59: 123-135. Full PDF Article

 

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