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【产品名称】
 EpiQuik Global Histone H3K9 Methylation Assay Kit
【订购货号】
 E158863
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-2-11  
Product Overview

The EpiQuik™ Global Histone H3-K9 Methylation Assay Kit is suitable for specifically measuring global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K9 METHYLATION ASSAY KIT?

Quick and efficient procedure, which can be finished within 5 hours. Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography. Strip microwell format makes the assay flexible: manual or high throughput. Simple, reliable, and consistent assay conditions.


Fig. 1. Measurement of H3-K9 methylation with different concentrations of the di-methyl H3-K9 standard from the EpiQuik™ Global Histone H3-K9 Methylation Assay Kit.

 

Product Details

The EpiQuik™ Global H3-K9 Methylation Assay Kit is designed for measuring global histone H3-K9 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K9 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:  


Product Components

GC1 (10X Lysis Buffer)
GC2 (Extraction Buffer)
GC3 (10X Wash Buffer)
GC4 (Histone Buffer)
GC5 (Blocking Buffer)
GC6 (Antibody Buffer)
GC7 (Capture Antibody, 100 µg/ml)*
GC8 (Detection Antibody, 400 µg/ml)*
GC9 (Developing Solution)
GC10 (Stop Solution)
Methylated H3-K9 Control (60 µg/ml)*
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

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