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【产品名称】
 EpiQuik In Situ Histone H3K4 Methylation Assay Kit
【订购货号】
 E158860
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-2-11  
Product Overview

The EpiQuik™ In Situ Histone H3-K4 Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K4 methylation in situ using cultured adherent cells.

WHY CHOOSE THE EPIQUIK™ IN SITU HISTONE H3-K4 METHYLATION ASSAY KIT?

Quick and efficient procedure, which can be finished within 3 hours. Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography. Measurement of in situ histone H3-K4 methylation without the need to prepare cell lysates. Microplate format makes the assay suitable for high throughput analysis of agents that increase or inhibit H3-K4 methylation. Simple, reliable, and consistent assay conditions. Product Details

EpiQuik™ In Situ Histone H3-K4 Methylation Assay Kit is a whole cell-based detection of methylated H3-K4. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The methylated H3-K4 is then detected by an anti-methyl H3-K4 antibody. The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:  


Product Components

GA1 (10X Wash Buffer)
GA2 (Permeabilizing Buffer)
GA3 (Blocking Buffer)
GA4 (Antibody Buffer)
GA5 (Capture Antibody, 100 µg/ml)*
GA6 (Detection Antibody, 200 µg/ml)*
GA7 (Developing Solution)
GA8 (Stop Solution)
30% H2O2 Solution
Methylated H3-K4 Control (20 µg/ml)
8-Well Control Strips
Microplates
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

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