北京启维益成科技有限公司 旗下网站

生物耗材网 请登录 | 免费注册 | 联系我们 
  首 页 >> 生物试剂 >> 细胞因子及蛋白 >> 重组蛋白 >>
【产品名称】
 IL-6 植物来源无动物成分
【订购货号】
 O377207
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-9-1  

IL-6, human, recombinant

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 185 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

 

Lot Number

 

01-1-002-7137

 

Available Sizes

 

10µg, 50µg, 100µg

 

Source

 

ISOkine™ recombinant human IL-6 is produced in the endosperm tissue of barley grain (Hordeum vulgare).

 

Formulation

 

Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2

 

Purity

 

Greater than 95% by SDS-PAGE gel analysis.

 

Reconstitution

 

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

 

Stability

 

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

Biological Activity

 

Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant human IL-6 was determined by its dose dependent effect on proliferation of 7TD-1 cells. The ED50 value for this effect is typically below 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

Endotoxin Level

 

Endotoxin level is less than 0.012ng per µg of ISOkine™ product (< 0.12EU/µg).

 

MAT assay

 

Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

 

Molecular Weight

 

Recombinant human IL-6 contains 185 amino acids and a 16 a.a. Histidine-based tag for a total length of 201 a.a. and has a predicted molecular mass of 23.1 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 27 kDa in SDS-PAGE.
打印此页】 【返回】【顶部】【关闭

【相关产品】