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  首 页 >> 生物试剂 >> 细胞因子及蛋白 >> 重组蛋白 >>
【产品名称】
 Activin A 植物来源无动物成分
【订购货号】
 O377194
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-9-1  
Activin A, human, recombinant
Activin A is a non-glycosylated cytokine that belongs to the TGFß superfamily. It is involved in a wide variety of biological processes including hematopoiesis, immune response, neural cell differentiation, wound repair and morphogenesis. The Activins form homodimers (e.g. AA; BB) and heterdimers (e.g. AB) and can activate the Smad pathway by signaling through heterodimeric typeI/typeII serine/threonine kinase transmembrane receptors. Activins are also important for production and regulation of several hormones such as FSH, Prolactin, ACTH and oxytocin. Activin A is synthesized by a wide spectrum of cells including bone marrow cells, skin cells, vascular smooth muscle cells and cells of the reproductive system.
Lot Number
0N-1-057-8115
Available Sizes
10µg, 50ug, 100ug
Source
Produced by transient expression in Nicotiana plant
Formulation
Lyophilized from Tris HCl at pH 7.4.
Purity
Greater than 95% by SDS page analysis.
Reconstitution
Lyophilized protein should be reconstituted in water to a suggested concentration of 25 µg/ml. Due to the protein nature, dimers and multimers may be observed. Not recommended to reconstitute in higher concentration than 25 µg/ml. At higher concentration the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
Stability
The lyophilized protein, though stable at 2-8°C in the short term, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Biological Activity
Bioactivity is assayed by measuring the ability of recombinant human Activin A to inhibit proliferation of mouse plasma-cytoma cell line (MPC-11). The ED50 value for this effect is typically below 5 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.05 EU/ug as measured by LAL method.
MAT assay
Molecular Weight
Dimer of 27,4 kDa. Recombinant human Activin A contains two protein chains of 116 amino acids each and a 6 aa Histidine-based tag for a total length of 122 aa.
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