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【产品名称】
 M-CSF 植物来源无动物成分
【订购货号】
 O377212
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-9-1  

M-CSF, human, recombinant

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological ativity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

 

Lot Number

 

01-1-022-7115

 

Available Sizes

 

10µg, 50µg, 100µg

 

Source

 

ISOkine™ recombinant human M-CSF is produced in the endosperm tissue of barley grain (Hordeum vulgare).

 

Formulation

 

Sterile filtered through a 0,2 µm filter. Lyophilized from PBS pH 7.2

 

Purity

 

Greater than 95% by SDS-PAGE gel analysis.

 

Reconstitution

 

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. The solution can be further diluted into aliquots in aquous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

 

Stability

 

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

Biological Activity

 

Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant human M-CSF was determined by its dose-dependent effect on the proliferation of M-NFS-60 cells. The ED50 value for this effect is typically < 2 ng/ml corresponding to specific activity > 5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

Endotoxin Level

 

Endotoxin level is less than 0.005ng per µg of ISOkine™ product (0.05EU/µg).

 

MAT assay

 

Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

 

Molecular Weight

 

Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE.
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