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【产品名称】
 Interleukin 4(IL-4) 植物来源无动物成分
【订购货号】
 O377206
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-9-1  

IL-4, human, recombinant

IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

 

Lot Number

 

01-1-005-8120

 

Available Sizes

 

10µg, 100µg, 1000µg

 

Source

 

ISOkine™ recombinant human IL-4 is produced in the endosperm tissue of barley grain (Hordeum vulgare).

 

Formulation

 

Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH7,2

 

Purity

 

Greater than 95% by SDS-PAGE gel analysis.

 

Reconstitution

 

Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile low salt buffer to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

 

Stability

 

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

Biological Activity

 

Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant human IL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically < 0.3 ng/ml, corresponding to a specific activity > 3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.

 

Endotoxin Level

 

Endotoxin level is less than 0.005ng per µg of ISOkine™ product (< 0.05EU/µg).

 

MAT assay

 

Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

 

Molecular Weight

 

Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. Due to glycosylation the recombinant protein migrates as smeary band with an apparent molecular mass of 23 kDa in SDS-PAGE.
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