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【产品名称】
 VEGF165 植物来源无动物成分
【订购货号】
 O377217
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2014-9-1  

VEGF165, human, recombinant

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

 

Lot Number

 

01-0-001-7139

 

Available Sizes

 

10µg, 50µg, 100µg, 1mg

 

Source

 

ISOkine™ recombinant human VEGF 165 is produced in the endosperm tissue of barley grain (Hordeum vulgare).

 

Formulation

 

Sterile filtered through a 0.2 µm filter. Lyophilized from PBS pH 7.2

 

Purity

 

Greater than 95% by SDS-PAGE gel analysis.

 

Reconstitution

 

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

 

Stability

 

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

Biological Activity

 

Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant human VEGF 165 was determined by its dose dependent effect on proliferation of Human Umbilical Vein Endothelial Cells (HUVEC). The ED50 for this effect is typically below 10 ng/ml corresponding to specific activity of > 1 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

Endotoxin Level

 

Endotoxin level is less than 0.005ng per µg of ISOkine™ product (< 0.05EU/µg).

 

MAT assay

 

Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

 

Molecular Weight

 

Recombinant human VEGF165 contains 165 amino acids and a 16 a.a. Histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 21.3 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26 - 28 kDa in SDS-PAGE.
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