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  首 页 >> 生物试剂 >> 抗体 >> 表观遗传抗体 >>
【产品名称】
 5-甲基胞嘧啶单克隆抗体
【订购货号】
 E16304
【产品CAS号码】
 无
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2015-4-2  

5-甲基胞嘧啶单克隆抗体描述、5-甲基胞嘧啶单克隆抗体浓度、5-甲基胞嘧啶单克隆抗体存储温度、5-甲基胞嘧啶单克隆抗体特性及其他说明

5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
5-甲基胞嘧啶(5-mC)单克隆抗体[33D3]
 
货号:A-1014
 
产品背景
DNA甲基化是真核生物基因组中的主要修饰途径,并在哺乳动物发育中起着重要作用。启动子区域DNA甲基化导致基因功能的失活。另外,根据组织类型不同DNA甲基化修饰形式不同,区域特异性DNA高甲基化和总DNA低甲基化已经证明在肿瘤形成中起着重要作用。
 
产品描述
针对5-甲基胞嘧啶(5-mC)的小鼠单克隆抗体,克隆33D3,MeDIP/ChIP-grade, 用于DNA甲基化研究。
 
浓度
1mg/ml
 
纯化形式
蛋白A
 
免疫原
卵清蛋白偶联的5-甲基胞嘧啶(5-mC)
 
同型对照
IgG1
 
特异性
修饰的基础5-甲基胞嘧啶(5-mC),反应物种广泛。
 
成分
存在于10mM磷酸盐缓冲液,150Mm NaCl,pH7.4的纯化IgG
 
储存
4℃可稳定保存6个月。长期储存的话,分成等份并储存于-20℃。避免反复冻融。多次冻融可能导致性能降低。
 
操作建议
为最大化回收产品,打开盖子之前请先离心。
 
别名
5-methylcytidine, anti-5-methylcytidine, anti-5-methylcytosine, anti-5mC, anti-5-mC, anti-5meC, anti-5-meC, 5mC, 5meC, 5-meC, 5'-methyl-2'-deoxycytidine, 5MedCyd
 
应用和建议稀释度*
Dot Blot: 1:1000-1:2000;
Immunohistochemistry: 1:100-1:500;
Immunofluorescence: 1:100-1:500;
ELISA: 1:1000-1:2000;
MeDIP: 0.5-1 µg/reaction
*最终使用者应根据实验摸索出最佳工作浓度。
 

Description
Mouse monoclonal antibody to 5-methylcytosine (5-mC), alternate name 5-methylcytidine, clone 33D3, ChIP-grade

Concentration
1 mg/ml

Specificity
Modified base 5-methylcytosine (5-mC), a broad range of species.

Isotype
IgG1

Formulation
10 mM phosphate buffer, 150 mM NaCl, pH 7.4

Storage
4°C, stable for 6 months from the date of shipment. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Application
WB: 1:200-1:500; IHC: 1:100-1: 500, ELISA: 1:1000-1:2000; IP: 2 µg/106 cells

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations


 

Ge, C. et. al. (March 2012). A simple colorimetric detection of DNA methylation. Analyst. Epub ahead of print. PubMed Abstract

Li, Y. et. al. (February 2012). IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages. Biochem Biophys Res Commun. Epub ahead of print. PubMed Abstract

Barra, V. et. al. (February 2012). Bypass of cell cycle arrest induced by transient DNMT1 post-transcriptional silencing triggers aneuploidy in human cells. Cell Div. Epub ahead of print. PubMed Abstract

Zhang, Y. et. al. (October 2011). Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells. J Exp Clin Cancer Res. 30(1):98. PubMed Astract

Aoi, Y. et. al. (October 2011). The involvement of DNA and histone methylation in the repression of IL-1ß-induced MCP-1 production by hypoxia. Biochem Biophys Res Commun. 414(1)252-8. PubMed Abstract

Li, L. et. al. (July 2011). Genome-wide DNA methylation patterns in IVF-conceived mice and their progeny: a putative model for ART-conceived humans. Reprod Toxicol. 32(1):98-105. PubMed Abstract

Liu, S. et. al. (March 2011). Effect of gonadotropins on dynamic events and global deoxyribonucleic acid methylation during in vitro maturation of oocytes: an animal model. Fertil Steril. 95(4): 1503-1506.e3. PubMed Abstract

Mandrioli, M. et. al. (January 2011). Composition and Epigenetic Markers of Heterochromatin in the Aphid Aphis nerii (Hemiptera: Aphididae). Cytogenet Genome Res. Epub ahead of print. PubMed Abstract

Yang, Y. et. al. (October 2010). Identification of methylated regions with peak search based on Poisson model from massively parallel methylated DNA immunoprecipitation-sequencing data. Electrophoresis. 31(21): 3537-44. PubMed Abstract 

Chen, X. et. al. (June 2010). Similar DNA Methylation and Histone H3 Lysine 9 Dimethylation Patterns in Tripronuclear and Corrected Bipronuclear Human Zygotes. J Reprod Dev. 56(3): 324-9. PubMed Abstract

Chen, C. et. al. (June 2009). Aberrant DNA methylation in thymic epithelial tumors. Cancer Investigation. 27(5): 582-91. PubMed Abstract

Roth, T. L. et. al. (May 2009). Lasting Epigenetic Influence of Early-Life Adversity on the BDNF Gene. Biol Psychiatry. 65(9): 760-9. Full PDF Article 

Tsuji, Y. et. al. (May 2009). The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2'-deoxycytidine. Zygote. 17(2): 109-15. PubMed Abstract

Weng, Y. I. et. al. (2009). Methylated DNA immunoprecipitation and microarray-based analysis: detection of DNA methylation in breast cancer cell lines. Methods Mol Biol. 590: 165-76. PubMed Abstract

Yun, X. X. et. al. (2009). DNA methylation patterns of mouse tetraploid embryos. Hereditas (Beijing). 31(4):387-392. Full PDF Article

Lubin, F. D. et. al. (October 2008). Epigenetic Regulation of bdnf Gene Transcription in the Consolidation of Fear Memory. J Neurosci.28(42): 10576-86. PubMed Abstract

Novikova, S. I. et. al. (April 2008). Maternal Cocaine Administration in Mice Alters DNA Methylation and Gene Expression in Hippocampal Neurons of Neonatal and Prepubertal Offspring. PLoS ONE. 3(4): e1919. Full PDF Article

Mandrioli, M. et. al. (November 2007). Analysis of heterochromatic epigenetic markers in the holocentric chromosomes of the aphid Acyrthosiphon pisum. Chromosome Research. 15(8): 1015-22. PubMed Abstract


 

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