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【产品名称】
 EpiSonic多功能生物处理器1000  
【订购货号】
 E14585
【规格/价格】
 
【预计到货时间】
 30 天
【产品详细说明】
【本产品仅供科学研究使用】
【更新日期】 2012-3-7  
Product Overview


The EpiSonic™ Multi-Functional Bioprocessor 1000 is part of the latest generation of sonication devices for use in a wide range of biological applications, including DNA and chromatin shearing for high throughput genome sequencing and next generation chromatin immunoprecipitation (ChIP). This completely digital instrument allows for rapid processing via 96 samples at a time and can be easily integrated into existing lab workflows.

Applications
DNA Shearing, Chromatin Shearing,
Cell Disruption, Cell Lysis, Nanoparticle Dispersion, Disaggregation, Homogenization, Cleaning, Mixing, Degassing, Dispersion, Cleavage, Catalyzing Reactions, Hydrolyzation, Extraction, Dissolution, Emulsification, Comminution, Synthesis, Microencapsulation

Features
As the most advanced ultrasonic processor, the EpiSonic 1000 has the following unique features:

  • Extremely simple operation through programmed digital processing and touch screen control
  • High degree of resolution through controlled output amplitude from 0-100%, allowing you to pinpoint the amount of energy needed to effectively process your sample
  • No risk of contamination -- ability to sonicate samples in sealed tubes or vials
  • True high throughput format -- process samples with 96 PCR tubes (0.2 ml) or a 96-well PCR plate.
  • Convenience and flexibility -- simultaneously sonicate 1 to 96 samples in a single run with volumes ranging from 10 µl to 2 ml
  • Very fast processing -- shearing 96 DNA samples to 200-600 bp fragments only needs 10-15 min
  • Improved bio-safety -- no aerosol formation and little foaming; no tedious manipulation of probes
  • Compatible with high-throughput ChIP and massively parallel sequencing workflows by using multi-channel pipetting and a 96 sample format

Specifications

 

 

Input Voltage 100 VAC - 240 VAC @ 50/60 Hz
Rated Current 100 VAC - 120 VAC, 7 Amps max.
220 VAC - 240 VAC, 3.5 Amps max.
Fuse Rating 8 Amps (Fast-Acting) for 100 VAC - 120 VAC
4 Amps (Fast-Acting) for 220 VAC - 240 VAC
Generator Weight 13.5 lbs (6.1 kgs)
Generator Dimensions 19.5"L x 11"W x 5.25"H (49.5 cm x 27.9 cm x 13.3 cm)
Sound Enclosure Weight 24.5 lbs (11.1 kgs)
Sound Enclosure Dimensions 10"L x 10"W x 16.5"H (25.5 cm x 25.5 cm x 42 cm)
Output Voltage 1000 V rms (max.)
Output Frequency 20 KHz (nom.)
Operating Environment Temperature: 39°-95°F (4°-35°C)
Relative Humidity 20-90% (Non Condensing)
For indoor use only
Shipping Environment Temperature: 35°-120°F (2°-49°C)
Relative Humidity 10-90% (Non Condensing)
Ambient Pressure Extremes: 40,000 ft. (12,000 meters)
Compliancy Restriction of Hazardous Substances (ROHS) Compliant, Directive 2002/95/EC
Regulatory Information CE, IEC 61010-1, CAN/CSA - C22.2 No. 61010-1 2nd Ed., IEC 61010-1, Mod

 

Product Details


Working With Next Generation ChIP & Genome Sequencing
The major features of next generation ChIP and genome sequencing are speed, efficiency, and high throughput capability -- rapid and massive generation of random DNA fragments in the optimized size is critical. There are several methods for fragmenting chromatin and DNA, which include enzymatic digestion and physical shearing such as probe sonication and nebulization. However, such methods have been too limited for next generation ChIP and massively parallel sequencing -- enzymatic digestion generates sequence-specific cleavage bias, while probe sonication and nebulization cause cross-contamination between the samples, inconsistent shearing, and fail to provide multiplexing capacity. As the most advanced shearing system, the EpiSonic 1000 avoids these problems and perfectly meets the requirements of next-generation ChIP and genome sequencing.

Chromatin Shearing With EpiSonic
For a successful ChIP, it is crucial to prepare the optimized target protein bound-DNA fragment (200-700 bp) and to maximize binding of antibody to the target protein. Using the EpiSonic 1000 and the included chromatin shearing protocol for ChIP, chromatin can be sheared to an optimal size range (200-700 bp) within just 15 minutes.

1-Hour ChIP With EpiSonic
The EpiSonic 1000 can be combined with the ChromaFlash One-Step ChIP Kit for true next generation chromatin immunoprecipitation. ChromaFlash is a next generation ChIP technology developed by Epigentek through the use of EpiSonic. It enables chromatin shearing and immunoprecipitation to be simultaneously carried out on the EpiSonic sonication system, greatly propelling the entire ChIP process to the fastest speeds in a high throughput format with high efficiency. This EpiSonic-based next generation ChIP technique, from chromatin to purified DNA, can be finished within 60 minutes in a 96 to 384-well format, which is extremely useful for high throughput mapping of global protein-DNA interactions through ChIP-Seq.

High Throughput Sequencing With EpiSonic
High demand for massive parallel analysis of genetic and epigenetic alterations has driven the development of high-throughput sequencing technologies that parallelize the sequencing process, producing thousands or millions of sequences at once.

An efficient, reliable method and device is needed to generate proper DNA fragments in a rapid and high throughput format in order to be compatible with high throughput sequencing. Epigentek’s EpiSonic is proven to be a powerful DNA shearing device and provides optimal fragment size, sample yield, consistency, speed, and high throughput capability, all of which are essential and compatible with massively parallel sequencing workflows including library construction. Some highlights include:

  • Optimal size range and consistency. DNA is sheared to the desired fragment range (200-600 bp)
  • Controlled DNA fragment size range. Because different downstream applications may need different fragment size ranges, the EpiSonic can generate different fragmentation ranges by digitally adjusting the sonication duration and output power.
  • High compatibility. Use of standard laboratory disposable tubes/vials; flexible range of number, volume, and type of various samples; compatible with downstream workflow steps and various high-throughput sequencing platforms.
  • High throughput format. Simultaneously sonicate 96 to 384 samples in a single run.


Fig. 1. Dynamic Chromatin Shearing | MDA-231 cells were fixed with 1% formaldehyde at 37°C for 10 mins. 1X 106 cells were lysed in 100 µl of sonication buffer and sonicated in a 0.2 ml PCR tube with the EpiSonic 1000. Each lane shows purified DNA fragments from sonication conditions of 20 sec. ON and 20 sec. OFF, 140 W with different cycles. Lanes 1 and 7: markers; Lane 2: unsonicated control; Lane 3: 5 cycles; Lane 4: 10 cycles; Lane 5: 15 cycles; Lane 6: 20 cycles.

Ideal DNA fragment size (200-600 bp) for ChIP was generated under sonication conditions of 20 cycles in 13 minutes.


Fig. 2. DNA Shearing | Placenta DNA in 100 µl of buffer was sonicated in a 0.2 ml PCR tube with the EpiSonic 1000. Lanes 1 and 7: markers; Lanes 2 and 3: DNA fragments from 1 µg and 2 µg DNA, respectively, using sonication conditions of 30 sec. ON and 30 sec. OFF, 120 W, 30 cycles; Lanes 4 and 5: DNA fragments from 1 µg and 2 µg DNA, respectively, using sonication conditions of 20 sec. ON and 20 sec. OFF, 120 W, 30 cycles; Lane 6: unsonicated control.

Ideal DNA fragments (100-500 bp, average 200-300 bp) for next-generation sequencing templates or library preparation can be generated from these sonication conditions.

 

  EpiSonic™ Competitor D Competitor C Probe Sonication Enzymatic Digestion
Desired Size Range of DNA Yes Yes Yes Yes No
High Yields of Unbiased dsDNA Yes Yes Yes No No
Number of Samples Per Run 1-96 1-48 1-96 1 N/A
Prevents Risk of Contamination Yes Yes Yes No N/A
Digital Readouts and Touchscreen Display Yes No No No No
Small Sample Volume Permitted (<25 µl) Yes Yes Yes No Yes
Simplicity & Convenience High Acceptable Acceptable Low Low
Fits Into Existing Lab Workflows Yes Yes No N/A No
Protocols Programmable Into Internal Memory Yes No No No No
Reproducibility Excellent N/A Good Poor Poor
Device Warranty 20 Months 12 Months 12 Months N/A N/A

 

Product Components


Digital Sonication Generator (Part #ECQ-1000-PSG)
Sample Processing Horn (Part #ECQ-1000-PPH)
Soundproof Enclosure Box (Part #ECQ-1000-PSE)
Ultrasonic Converter (Part #ECQ-1000-PUC)
Converter Cable (Part #ECQ-1000-PCC)
Water Flow Tubing (Part #ECQ-1000-PFT)
Power Cord (Part #ECQ-1000-PPC)
Assembly Wrenches (Part #ECQ-1000-PAW)

User Guide & MSDS


[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

 

Catalog No.+ Product Name   Price
EQC-1000 EpiSonic Multi-Functional Bioprocessor 1000    
EQC-1000-ATP EpiSonic Temperature Probe   RMB3200.00
EQC-1000-X02 EpiSonic 0.2 ml PCR Vials (100 count)   RMB520.00
EQC-1000-X96 EpiSonic 96-Well PCR Plate (5/pack)   RMB800.00
EQC-1000E-PTR EpiSonic 0.2 ml Tube Rack   RMB3200.00

 

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